Product Profile

Product Name

Rabbit anti-ANXA1 polyclonal antibody - N-terminal region

Antibody Type

Primary Antibodies

Immunogen

The immunogen for anti-ANXA1 antibody: synthetic peptide directed towards the N terminal of human ANXA1

Key Feature

Clonality

Polyclonal

Isotype

IgG

Host Species

Rabbit

Tested Applications

WBIHC

WB:1:500~1:2000
IHC:1:50~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Species Reactivity

BovineDogGuinea PigHumanMousePig

Concentration

1 mg/ml

Purification

Protein A

Target Information

Gene Symbol

ANXA1

Gene Synonyms

ANX1
LPC1

Gene Full Name

Annexin A1

Gene Summary

More

ANXA1 encodes a protein that belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

Alternative Names

ANX1
LPC1

Molecular Weight(MW)

38kDa

Sequence

346 amino acids

Database Links

Entrez Gene

301

SwissProt ID

Q5TZZ9

Protein Accession

NP_000691

Application

Immunohistochemical

Immunohistochemical

Western blot

Western blot

  • Host: Rabbit
    Target Name:ANXA1
    Sample Tissue:HepG2
    Lane A: Primary Antibody
    Lane B: Primary Antibody + Blocking Peptide
    Primary Antibody Concentration:2.5ug/mL
    Peptide Concentration: 2.0ug/mL
    Lysate Quantity: 25ug/lane
    Gel Concentration: 12%

Western blot

Western blot

  • Positive Control: Lane 1: 20ug mouse fibroblast lysates
    Primary Antibody Dilution : 1:600
    Secondary Antibody : Anti rabbit-HRP
    Secondry Antibody Dilution : 1:2000
    Submitted by: Anonymous

Western blot

Western blot

  • 1.25ug/ml
    ELISA Titer: 1:62500
    Positive Control: HepG2 cell lysate

Application Notes

WB:1:500~1:2000
IHC:1:50~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form

Liquid

Storage Instructions

Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles

Storage Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.