范潇博士全职加入深圳医学科学院|em|博士|实验|深圳医学科学院|电镜|膜蛋白|范潇

来源：深圳医学科学院

个人简介

范潇博士于 2014 年获清华大学学士学位，2019 年获清华大学博士学位。2019 年至 2025 年间，先后于普林斯顿大学及洛克菲勒大学/霍华德 · 休斯医学研究所（HHMI）从事博士后研究。2025 年 8 月全职加入深圳医学科学院，任生物构造与互作研究所特聘研究员。

范潇博士长期致力于融合多学科前沿技术方法，以推动冷冻电镜技术创新，并聚焦于重大疾病相关膜蛋白-药物分子相互作用机制的结构生物学研究。通过自主研发的冷冻电镜全流程创新技术体系，克服了该领域长期存在的技术瓶颈，成功实现了超小分子量蛋白及近生理状态下膜蛋白的高分辨率结构解析，并揭示了电压门控钠离子/钙离子通道的结构与药理作用机制等一系列重要发现。

主要奖项与荣誉

人类前沿科学计划 (HFSP) 博士后基金奖

课题组研究方向

范潇课题组将立足于多学科交叉领域，发挥技术优势，开发新型冷冻电镜成像技术及高效样品制备与数据处理方法，构建针对天然膜体系的膜蛋白原位结构与功能解析平台。并将依托该平台开展复杂生物膜环境的高效原位研究，通过多维度解析关键大分子的空间构型、相互作用网络及跨尺度组织模式，深入探索生物膜系统间的耦合与调控机制，为相关疾病干预提供理论基础。

主要论文集

* Equal contributions # Corresponding author

主要论文集：

1.Xiao Fan*, Jiaofeng Chen*, Xiaoshuang Huang, Zhanfeng Hou, Yuzhen Xie, Zigang Li, Nieng Yan#, and Jian Huang# (2025). Phrixotoxin-3 inhibits human Nav1.6 through tri-pocket binding. Cell Research, 35(8):610-613.

2.Zi Yang*, Xiao Fan*, and Hongwei Wang# (2025). Near-atomic resolution single-particle 3D reconstruction using a single Cryo-EM micrograph. Journal of Chinese Electron Microscopy Society, 44 (1): 1000-6281

3.Jian Huang*, Xiao Fan*, Xueqin Jin, Chen Lyu, Qinmeng Guo, Tao Liu, Jiaofeng Chen, Amaël Davakan, Philippe Lory, and Nieng Yan# (2024). Structural basis for human Cav3.2 inhibition by selective antagonists. Cell Research, 0:1–11.

4.Yang, Zi, Jingjin Fan, Jia Wang, Xiao Fan, Zheng Ouyang, Hong-Wei Wang#, and Xiaoyu Zhou# (2024). Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nature Methods: 1-10.

5.Kristina Solorio-Kirpichyan, Xiao Fan*, Dmitrij Golovenko*, Andrei A. Korostelev, Nieng Yan, and Alexei Korennykh# (2024). Cryo-EM structure of histidyl-tRNA synthetase-like domain reveals activating crossed helices at the core of GCN2. PNAS nexus 3, no. 12: pgae528.

6.Xiao Fan*#, Jian Huang*, Xueqin Jin and Nieng Yan# (2023). Cryo-EM structure of human voltage-gated sodium channel Nav1.6. Proceedings of the National Academy of Sciences, 120(5), e2220578120.

7.Jian Huang*, Xiao Fan*#, Xueqin Jin, Liming Teng and Nieng Yan#. (2023). Dual-pocket inhibition of Nav channels by the antiepileptic drug lamotrigine. Proceedings of the National Academy of Sciences, 120(41), e2309773120.

8.Qiurong Wu*, Jian Huang*#, Xiao Fan*#, Gaoxingyu Huang, Xueqin Jin, Xiaojing Pan# and Nieng Yan# (2023). Structural mapping of Nav1.7 antagonists. Nature Communications, 14 (1), 3224.

9.Jian Huang*, Xiao Fan*, Xueqin Jin, Sooyeon Jo, Hanxiong Bear Zhang, Akie Fujita, Bruce P. Bean#, and Nieng Yan# (2023). Cannabidiol inhibits Nav channels through two distinct binding sites. Nature Communications, 14(1), 3613.

10.Shuai Gao, Xia Yao, Jiaofeng Chen, Gaoxingyu Huang, Xiao Fan, Lingfeng Xue, Zhangqiang Li, Tong Wu, Yupeng Zheng, Jian Huang, Xueqin Jin, Yan Wang, Zhifei Wang, Yong Yu, Lei Liu, Xiaojing Pan, Chen Song and Nieng Yan. (2023). Structural basis for human Cav1.2 inhibition by multiple drugs and the neurotoxin calciseptine. Cell, 186(24), 5363-5374.

11.Peng Huang, Raminta Venskutonytė, Rashmi B. Prasad, Hamidreza Ardalani, Sofia W. de Maré, Xiao Fan, Ping Li, Peter Spégel, Nieng Yan, Pontus Gourdon, Isabella Artner and Karin Lindkvist-Petersson (2023). Cryo-EM structure supports a role of AQP7 as a junction protein. Nature Communications, 14(1), 600.

12.Xia Yao, Yan Wang, Zhifei Wang, Xiao Fan, Di Wu, Jian Huang, Alexander Mueller, Sarah Gao, Miaohui Hu, Carol V. Robinson, Yong Yu, Shuai Gao, and Nieng Yan (2022). Structures of the R-type human Cav2.3 channel reveal conformational crosstalk of the intracellular segments. Nature communications, 13(1): 7358.

13.Xia Yao, Shuai Gao, Jixin Wang, Zhangqiang Li, Jian Huang, Yan Wang, Zhifei Wang, Jiaofeng Chen, Xiao Fan, Weipeng Wang, Xueqin Jin, Xiaojing Pan, Yong Yu, Armando Lagrutta, Nieng Yan (2022). Structural basis for the severe adverse interaction of sofosbuvir and amiodarone on L-type Cav channels. Cell, 185(25), 4801-4810.

14.Jiemin Shen*, Miaohui Hu*, Xiao Fan*#, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming Zhou# (2022). Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport. Structure, 7(30): 1035-1041.e3.

15.Heng Ni*, Xiao Fan*, Feng Zhou, Galio Guo, Jae Young Lee, Nadrian C Seeman, Do-Nyun Kim, Nan Yao, Paul M Chaikin, and Yimo Han# (2022). Direct visualization of floppy two-dimensional DNA origami using cryogenic electron microscopy. iScience, 6(25): 104373.

16.Xiao Fan (2022). "Breaking down the barriers in cryo-EM analysis of membrane proteins." Foundations of Crystallography, 2022, 78: A240-A240.

17.Xia Yao*, Xiao Fan*#, Nieng Yan# (2020). Cryo-EM analysis of a membrane protein embedded in the liposome. Proceedings of the National Academy of Sciences, 117(31): 18497-18503.

18.Yimo Han*, Xiao Fan*, Haozhe Wang, Fang Zhao, Christopher Tully, Jing Kong, Nan Yao and Nieng Yan# (2020). High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy. Proceedings of the National Academy of Sciences, 117, 1009-1014.

19.Yaping Pan, Zhenning Ren, Shuai Gao, Jiemin Shen, Lie Wang, Zhichun Xu, Ye Yu, Preetham Bachina, Hanzhi Zhang, Xiao Fan, Arthur Laganowsky, Nieng Yan & Ming Zhou (2020). Structural basis of ion transport and inhibition in ferroportin. Nature communications 11(1), 1-11.

20.Xiao Fan*, Jia Wang*, Xing Zhang, Zi Yang, Jin-Can Zhang, Lingyun Zhao, Hai-Lin Peng, Jianlin Lei#, and Hong-Wei Wang# (2019). Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nature communications 10, 2386.

21.Hong-Wei Wang and Xiao Fan (2019). Challenges and opportunities in cryo-EM with phase plate. Current Opinion in Structural Biology, 58, 175–182.

22.Xiao Fan*, Lingyun Zhao*, Chuan Liu, Jin-Can Zhang, Kelong Fan, Xiyun Yan, Hai-Lin Peng, Jianlin Lei# and Hong-Wei Wang# (2017). Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM. Structure 25, 1623-1630.

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