Product Profile
Product Name
DLK1 Antibody
Antibody Type
Primary Antibodies
Product description
More
DLK1 Antibody: The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes ａnd is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal ａnd paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child ａnd adolescent obesity.1) Smas CM ａnd Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993; 73:725-34.
Immunogen
DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1. The immunogen is located within amino acids 260 - 310 of DLK1.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAICCIFWB
DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.:
Species Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Symbol
DLK1
Alternative Names
More
DLK1 Antibody: DLK
Protein delta homolog 1
pG2
DLK-1
Molecular Weight(MW)
42 kDa
Sequence Similarities
2) Cat. No. 17-011 - Hep G2 Cell Slide
Tissue Specificity
At least four isoforms of DLK1 are known to exist.
Database Links
Entrez Gene
8788
Protein Accession
NP_003827
Application
Application
- Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 ａnd (B) 2 μg/mL.
Application
- Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μg/ml.
Application
- Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μg/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
DLK1 antibody can be stored at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
DLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
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