Product Profile
Product Name
NQO1 Antibody (Center)
Antibody Type
Primary Antibodies
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
WBFCIF
WB~~1:50~100 FC~~1:10~50 IF~~1:10~50:
Species Reactivity
Human
Concentration
1 mg/ml
Purification
Target Information
Gene Synonyms
DIA4
NMOR1
Alternative Names
More
NQO1
DIA4
NMOR1
NAD(P)H dehydrogenase [qui] 1
Azoreductase
DT-diaphorase
Menadione reductase
NAD(P)H:qui oxidoreductase 1
Phylloqui reductase
Qui reductase 1
Molecular Weight(MW)
30868 Da
Function
The enzyme apparently serves as a qui reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis
Tissue Specificity
This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the Center region of human NQO1.
Cellular Localization
Cylasm.
Database Links
Entrez Gene
1728
Application
Application
- Western blot analysis of NQO1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.
Application
- NQO1 antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
- Confocal immunofluorescent analysis of NQO1 Antibody (Center)(Cat#AP7350c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application Notes
WB~~1:50~100 FC~~1:10~50 IF~~1:10~50:
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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