Product Profile

Product Name

Occludin

Antibody Type

Primary Antibodies

Product description

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This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration ａnd polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.

Immunogen

Synthetic peptide within human Occludin aa 1-50 conjugated to Keyhole Limpet Haemocyanin (KLH).

Key Feature

Clonality

Polyclonal

Isotype

IgG

Host Species

Rabbit

Tested Applications

WBICCIHCFC

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WB:1:500-2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Species Reactivity

HumanMouseRat

Concentration

1 mg/mL.

Target Information

Alternative Names

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BLCPMG antibody FLJ08163 antibody FLJ18079 antibody FLJ77961 antibody FLJ94056 antibody MGC34277 antibody Occludin antibody Ocln antibody OCLN_HUMAN antibody Phosphatase 1 regulatory subunit 115 antibody PPP1R115 antibody PTORCH1 antibody Tight junction protein occludin antibody

Molecular Weight(MW)

59 kDa

Cellular Localization

Cell membrane.

Application

Application

Application

  • Fig1: Western blot analysis of Occludin on mouse kidney (1) ａnd human kidney (2) tissue lysates using anti-Occludin antibody at 1/1,000 dilution.

Application

Application

  • Fig2: ICC staining Occludin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Application

  • Fig3: ICC staining Occludin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Application

  • Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.

Application

Application

  • Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.

Application

Application

  • Fig6: Flow cytometric analysis of HepG2 cells with Occludin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control

mouse kidney ａnd human kidney tissue lysates, HepG2, Hela, A549, human lung tissue, mouse lung tissue.

Application Notes

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WB:1:500-2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form

Liquid

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.