Product Profile
Product Name
GCLM antibody
Antibody Type
Primary Antibodies
Product description
More
Rabbit Polyclonal antibody to GCLM (glutamate-cysteine ligase, modifier subunit)Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit ａnd a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human GCLM. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFWB
*Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
HumanMouseRat
Predicted Reactivity
BovineBovine (96%)
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
More
GLCLR antibody
GCLM antibody
GSC light chain antibody
gamma-ECS regulatory subunit antibody
GCS light chain antibody
"glutamate-cysteine ligase (gamma-glutamylcysteine synthetase)
regulatory (30.8kD) antibody"
glutamate－－cysteine ligase modifier sub
Molecular Weight(MW)
31 kDa (note) (2)
Application
Application
- Sample (50 µg of whole cell lysate) A: rat liver 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
- Sample (30 µg of whole cell lysate) A: A431 12% SDS PAGE GCLM antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
- Immunofluorescence analysis of methanol-fixed HeLa, using GCLM antibody at 1:200 dilution.
Application
- Non-transfected (–) ａnd transfected (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
- Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
- Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Positive Control
HeLa , A431 , mouse liver , rat liver , rat kidney
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
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