Product Profile
Product Name
GATA6 antibody
Antibody Type
Primary Antibodies
Product description
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Rabbit polyclonal antibody to GATA6This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation ａnd organogenesis during vertebrate development. This gene is expressed during early embryogenesis ａnd localizes to endo- ａnd mesodermally derived cells during later embryogenesis ａnd thereby plays an important role in gut, lung, ａnd heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012]
Immunogen
Synthetic peptide sequence KYSGQDGLYIGVSLASPAE & KRFGAAGADASDSRAFPARE
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFIHCIHC-PWB
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ICC/IF:1:200~1:500
IHC:1:50~1:200
IHC-P:1:50~1:200
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouse
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
GATA binding protein 6
Target
GATA6
Application
Application
- IHC analysis of human stomach tissues with (right) or without (left) GATA6 antibody at a dilution of 1:100. To expose target protein, antigen was retreived using 10Mm sodium citrate followed by microwave treatment for 8-15 minutes.
Application
- ICC/IF analysis of HeLa cells using GATA6 antibody at a dilution of 1:200 (green). Nuclei (blue) were stained with Hoechst 33342 dye.
Application
- WB analysis of various cell lysates (25 ug per lane) using GATA6 antibody at a dilution of 1:1000.
Application Notes
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ICC/IF:1:200~1:500
IHC:1:50~1:200
IHC-P:1:50~1:200
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
-20° C
Storage Buffer
PBS with 1mg/ml BSA, 30% glycerol
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