Product Profile

Product Name

GRP78 antibody

Antibody Type

Primary Antibodies

Product description

Rabbit Polyclonal antibody to GRP78GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER pr

Immunogen

Synthetic peptide made to an internal portion of the human protein (within residues 250-300). [Swiss-Prot# P11021]

Key Feature

Clonality

Polyclonal

Isotype

IgG

Host Species

Rabbit

Tested Applications

FACSICC/IFWB

Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.:

Species Reactivity

HumanMouseChickenRatSheep

Predicted Reactivity

Bovine, C. elegans, Drosophila, Primate, ZebrafishPrimate (100%), Bovine (100%), Zebrafish (100%), Drosophila (100%), C. elegans (100%)

Concentration

1 mg/ml

Purification

Affinity purified

Target Information

Gene Synonyms

More

BIP
FLJ26106
MIF2
GRP78

Target

HSPA5

Tissue Specificity

Grp78

Cellular Localization

Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Application

Application

Application

  • Simple Western: GRP78/HSPA5 Antibody [ - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Application

Application

  • Detection of Bip/Grp78 on HeLa whole cell extracts using

Application Notes

Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.:

Additional Information

Form

Liquid

Storage Instructions

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Storage Buffer

PBS, 30% glycerol and 0.1% sodium azide was added as a preservative.