Product Profile

Product Name

WHSC2 Antibody

Antibody Type

Primary Antibodies

Product description

More

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, ａnd may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted ａnd tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Immunogen

This WHSC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-279 amino acids from the Central region of human WHSC2.

Key Feature

Clonality

Polyclonal

Isotype

Ig

Host Species

Rabbit

Tested Applications

WB

For WB starting dilution is: 1:1000:

Species Reactivity

Human

Concentration

1 mg/ml

Purification

Affinity purified

Target Information

Gene Symbol

NELFA

Alternative Names

More

Negative elongation factor A
NELF-A
Wolf-Hirschhorn syndrome candidate 2 protein
NELFA
WHSC2

Molecular Weight(MW)

57 kDa

Sequence Similarities

Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene

7469

Protein Accession

Q9H3P2

Application

Application

Application

  • Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

Application Notes

For WB starting dilution is: 1:1000:

Additional Information

Form

Liquid

Storage Instructions

Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.