Product Profile
Product Name
SLC27A5 [C10-B11]
Antibody Type
Primary Antibodies
Product description
More
Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine ａnd taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBIHC
WB:1:250~1:1000
IHC:1:20~1:100
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouse
Concentration
2 mg/mL.
Target Information
Alternative Names
More
ACSB antibody ACSVL6 antibody BA CoA ligase antibody BA-CoA ligase antibody BACS antibody BAL antibody Bile acid CoA ligase antibody Bile acid-CoA ligase antibody Bile acyl CoA synthetase antibody Bile acyl-CoA synthetase antibody Cholate CoA ligase antibody CholateCoA ligase antibody FACVL3 antibody FATP 5 antibody FATP-5 antibody FATP5 antibody Fatty acid Coenzyme A ligase very long chain 3 antibody Fatty acid transport protein 5 antibody Fatty-acid-coenzyme A ligase antibody FLJ22987 antibody S27A5_HUMAN antibody Slc27a5 antibody Solute carrier family 27 (fatty acid transporter) member 5 antibody Solute carrier family 27 member 5 antibody Very long chain acyl CoA synthetase homolog 2 antibody Very long chain acyl CoA synthetase related protein antibody very long-chain 3 antibody Very long-chain acyl-CoA synthetase homolog 2 antibody Very long-chain acyl-CoA synthetase-related protein antibody VLACS related antibody VLACS-related antibody VLACSR antibody VLCS H2 antibody VLCS-H2 antibody VLCSH2 antibody
Molecular Weight(MW)
75 kDa
Cellular Localization
Endoplasmic reticulum membrane.
Database Links
SwissProt ID
Q9Y2P5
Application
Application
- Fig1: Western blot analysis of SLC27A5 on human SLC27A5 recombinant protein using anti-SLC27A5 antibody at 1/1,000 dilution.
Application
- Fig2: Western blot analysis of SLC27A5 on HEK293 (1) ａnd SLC27A5-hIgGFc transfected HEK293 (2) cell lysate using anti-SLC27A5 antibody at 1/1,000 dilution.
Application
- Fig3: Western blot analysis of SLC27A5 on 3T3L1 (1) ａnd NIH3T3 (2) cell lysate using anti-SLC27A5 antibody at 1/1,000 dilution.
Application
- Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SLC27A5 antibody. Counter stained with hematoxylin.
Application
- Fig5: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-SLC27A5 antibody. Counter stained with hematoxylin.
Positive Control
Human SLC27A5 recombinant protein, SLC27A5-hIgGFc transfected HEK293 cell lysate, 3T3L1, NIH3T3 cell lysate, human liver cancer tissue, human stomach tissue.
Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
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