Product Profile
Product Name
ANXA1 Antibody
Antibody Type
Primary Antibodies
Product description
More
ANXA1 encodes a protein that belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 ａnd are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins ａnd leukotrienes, annexin I may have potential anti-inflammatory activity.1) Ernst, S., et al., (2004) J. Immunol. 172 (12), 7669-7676.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity
Human
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
ANXA1
Alternative Names
ANXA1
ANX1
LPC1
Molecular Weight(MW)
38 kDa
Database Links
Entrez Gene
301
Protein Accession
NP_000691
Application
Application
- Antibody used in WB on mouse fibroblasts at 1:600.
Application
- Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody ａnd Lane B: Primary Antibody + Blocking Peptide ).
Application
- Antibody used in IHC on Human Intestine.
Application
- Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
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