Product Profile
Product Name
Annexin A1 Antibody
Antibody Type
Primary Antibodies
Product description
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Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 ａnd are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins ａnd leukotrienes, annexin I may have potential anti-inflammatory activity.1) Shimoji,T., J. Cell. Biochem. 106 (6), 1123-1135 (2009)
Immunogen
This Annexin A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the Central region of human Annexin A1.
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
FACSIHC-PWB
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity
Human
Concentration
1 mg/ml
Target Information
Gene Symbol
ANXA1
Alternative Names
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Annexin A1
Annexin I
Annexin-1
Calpactin II
Calpactin-2
Chromobindin-9
Lipocortin I
Phospholipase A2 inhibitory protein
p35
ANXA1
ANX1
LPC1
Molecular Weight(MW)
39 kDa
Database Links
Entrez Gene
301
Protein Accession
P04083
Application
Application
- Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
- Western blot analysis of Annexin A1 antibody in A2058 cell line lysates (35ug/lane)
Application
- Annexin A1 Antibody IHC analysis in formalin fixed ａnd paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.
Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
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