Product Profile

Product Name

PPM1A Antibody (Center)

Antibody Type

Primary Antibodies

Key Feature

Clonality

Polyclonal

Isotype

Ig

Host Species

Rabbit

Tested Applications

WBIHC

WB~~1:100~500 IHC~~1:50~100:

Species Reactivity

Human

Concentration

1 mg/ml

Purification

Target Information

Gene Synonyms

PPPM1A

Alternative Names

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PPM1A
PPPM1A
Protein phosphatase 1A
Protein phosphatase 2C isoform alpha
Protein phosphatase IA

Molecular Weight(MW)

42448 Da

Function

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 ａnd SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs ａnd termination of the TGF-beta-mediated signaling

Tissue Specificity

This PPM1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64~94 amino acids from the center region of human PPM1A.

Cellular Localization

Nucleus.

Database Links

Entrez Gene

5494

Application

Application

Application

  • Western blot analysis of hPPM1A-C72 (RB06012) in HL-60 cell line lysates (35ug/lane).PPM1A (arrow) was detected using the purified Pab.

Application

Application

  • Western blot analysis of PPM1A (arrow) using rabbit polyclonal hPPM1A-C72 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1A gene (Lane 2) (Origene Technologies).

Application

Application

  • Formalin-fixed ａnd paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Application Notes

WB~~1:100~500 IHC~~1:50~100:

Additional Information

Form

Liquid

Storage Instructions

For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Storage Buffer

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.