Product Profile

Product Name

EIF4B Antibody

Antibody Type

Primary Antibodies

Product description

More

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F ａnd EIF4-A by binding near the 5＇- terminal cap of mRNA in presence of EIF-4F ａnd ATP. This gene also promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F

Immunogen

This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-382 amino acids from the Central region of human EIF4B.

Key Feature

Clonality

Polyclonal

Isotype

Ig

Host Species

Rabbit

Tested Applications

IHC-PWB

For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Species Reactivity

Human

Concentration

1 mg/ml

Target Information

Gene Symbol

EIF4B

Alternative Names

Eukaryotic translation initiation factor 4B
eIF-4B
EIF4B

Molecular Weight(MW)

69 kDa

Sequence Similarities

Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene

1975

Protein Accession

P23588

Application

Application

Application

  • Formalin-fixed ａnd paraffin-embedded human breast carcinoma reacted with EIF4B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Application

Application

  • Western blot analysis of anti-EIF4B Antibody Pab in HepG2 cell line lysates (35ug/lane).

Application

Application

  • Western blot analysis of anti-EIF4B Antibody Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane).

Application

Application

  • Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes

For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Additional Information

Form

Liquid

Storage Instructions

Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.