Product Profile

Product Name

eIF4B Antibody

Antibody Type

Primary Antibodies

Product description

More

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F ａnd EIF4-A. Binds near the 5＇-terminal cap of mRNA in presence of EIF-4F ａnd ATP. Promotes the ATPase activity ａnd the ATP-dependent RNA unwinding activity of both EIF4-A ａnd EIF4-F.1) Gingras, A. C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. ａnd Hershey, J. W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R. F. ａnd Hershey, J. W. (1989) J. Cell Biol. 109, 1467-1481.

Immunogen

eIF4B (Ab-422) antibody was raised against a peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.

Key Feature

Clonality

Polyclonal

Host Species

Rabbit

Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Species Reactivity

Human

Concentration

1 mg/ml

Purification

Affinity purified

Target Information

Gene Symbol

EIF4B

Alternative Names

More

EIF-4B
PRO1843
PKB
RAC

Molecular Weight(MW)

80 kDa

Tissue Specificity

This antibody detects endogenous level of total eIF4B protein.

Database Links

Entrez Gene

1975

Protein Accession

NP_001408.2

Application

Application

Application

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B (Ab-422).

Application

Application

  • Western blot analysis of lysed extracts from 3T3 ａnd HL60 cells using eIF4B (Ab-422).

Application Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form

Liquid

Storage Instructions

Store antibody at -20˚C for up to one year.

Storage Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.