Product Profile
Product Name
PPP2R4 [G4-D9]
Antibody Type
Primary Antibodies
Product description
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n general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit ａnd a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) ａnd PP2C. PPP2R4 (protein phosphatase 2A activator, regulatory subunit 4), also known as PR53 or PTPA, is a 358 amino acid protein that is widely expressed ａnd associates with the PP holoenzyme. Specifically, PPP2R4 functions to stimulate the ATP- ａnd magnesium-dependent phosphotyrosyl phosphatase activity of the dimeric form of PP2A, thereby affecting the control of cell growth ａnd division. Four isoforms of PPP2R4, designated alpha, beta, delta ａnd epsilon, are expressed due to alternative splicing events.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBICCIHCFC
More
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
Species Reactivity
Human
Concentration
2 mg/mL.
Target Information
Alternative Names
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subunit B antibody KIAA0044 antibody MGC2184 antibody Phosphotyrosyl phosphatase activator antibody PP2A antibody PP2A phosphatase activator antibody PP2A subunit B antibody PP2A subunit B isoform PR53 antibody PP2A subunit B PR53 isoform antibody PPP2R4 antibody PR53 antibody PR53 isoform antibody Protein phosphatase 2
regulatory subunit B-prime antibody Protein phosphatase 2A activator regulatory subunit 4 antibody Protein phosphatase 2A regulatory subunit B＇ (PR 53) antibody PTPA antibody PTPA_HUMAN antibody Serine/threonine protein phosphatase 2A regulatory subunit B antibody Serine/threonine-protein phosphatase 2A activator antibody Serine/threonine-protein phosphatase 2A regulatory subunit 4 antibody Serine/threonine-protein phosphatase 2A regulatory subunit B antibody
Molecular Weight(MW)
41 kDa
Cellular Localization
Cylasm. Nucleus.
Database Links
SwissProt ID
Q15257
Application
Application
- Fig1: Western blot analysis of PPP2R4 on human PPP2R4 recombinant protein using anti-PPP2R4 antibody at 1/1,000 dilution.
Application
- Fig2: ICC staining PPP2R4 (green) ａnd actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
- Fig3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.
Application
- Fig4: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.
Application
- Fig5: Flow cytometric analysis of MCF-7 cells with PPP2R4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human PPP2R4 recombinant protein, Hela, cervical cancer tissue, lung cancer tissue, MCF-7.
Application Notes
More
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
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