Syd Labs体内实验级重组大鼠IgG2b同型对照抗体（In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）。
重组大鼠IgG2b kappa同型对照（Recombinant rat IgG2b kappa isotype control）和Fc effector 大鼠IgG2b kappa同型对照（rat IgG2b kappa isotype control）均适用于体外和体内研究。低或无特异性结合小鼠样本测试。小鼠可变区和大鼠IgG2b kappa恒定区。
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货号：PA007144
英文名：In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
供货商名称：Syd Labs, Inc.
公司名：Syd Labs
别称：重组大鼠IgG2b同型对照抗体，大鼠IgG2b阴性对照抗体，rtIgG2b同型对照抗体
概述：用哺乳动物细胞生产的体内实验级重组大鼠IgG2b同型对照抗体，（In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）。
克隆号：12B9
同种型：大鼠 IgG2b, kappa
应用：ELISA、Western Blot (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究中对大鼠IgG2b kappa抗体进行同型匹配阴性对照。
免疫源：N/A
抗体形式：0.2 μM过滤溶液，1x PBS
内毒素：根据 LAL 方法，≤1 EU每1mg 蛋白质。提供特级体内实验级重组大鼠IgG2b同型对照抗体（In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody，内毒素≤0.05 EU/mg)。
纯度：>95%（在还原条件下通过SDS-PAGE测定）
运输：Syd Labs体内实验级重组大鼠IgG2b同型对照抗体（In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）用冰袋运输。收到后，请立即将其存放在下面建议的温度下。
稳定性与存储：使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，如果保存在2 至 8°C。 自收到之日起 3 个月，如果保存在-20 至 -70°C。
注意事项：最便宜的体内实验级重组人IgG1 kappa同型对照抗体和突变体（In Vivo Grade Recombinant Human IgG1 kappa Isotype Control Antibody mutant）;与经测试的人体样本的特异性结合程度低或无特异性结合;人源化可变区和人IgG1 kappa恒定区;可提供嵌合版本和优质级(内毒素≤0.05 EU/mg)。
PA007144：体内实验级重组大鼠IgG2b同型对照抗体（In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）
背景知识
Rat IgG2b isotype control and mutants（大鼠IgG2b同型对照和突变体） (natural and engineered rtIgG2b isotype controls) are useful for functional study using animal model among a number of in vitro and in vivo research applications. Rat IgG2b antibodies are more like mouse IgG2a and IgG2b, and human IgG1 antibodies, which are mostly used by the therapeutic antibody drugs. Most of rat antibodies are used to study mouse target proteins.
There are more and more IgG antibody formats with Fc engineered. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular. We provide Fc effector-silent rat IgG2b isotype controls（大鼠IgG2b同型对照）.
Please contact us to ask for a quote for the engineered rat IgG2b isotype control mutants（大鼠IgG2b同型对照突变体）, rtIgG2b isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated rtIgG2b isotype control and mutants（rtIgG2b同型对照和突变体）. A variety of conjugates (such as dyes and fluorophores) with the rat IgG2b isotype control（大鼠IgG2b同型对照） and Fc mutants are available.
Rat IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a, IgG2b, and IgG2c for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.
问答:
Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate any non-specific background signal from the specific antibody signal. Different from our recombinant rat IgG2b isotype control（重组大鼠IgG2b同型对照） (catalog number PA007144), other rat IgG2b kappa isotype controls（大鼠IgG2b kappa同型对照） against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity.
Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage for the in vitro method. First, we do not use mouse ascites for antibody production. Rat hybridoma cell lines usually adapt poorly to in vitro conditions, and generate ascites in immunocompromised mice rather than rats. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies. Fourth, we can easily switch the isotype with different antibody constant regions, and generate mutations such as LALAPG which is used in therapeutic antibody research. It is better to use the same isotype control mutation.
Question: Do you use the antibody sequences of MOPC21 (hybridoma clone number, isotype: mouse IgG1, kappa) or C1.18.4 (hybridoma clone number, isotype: mouse IgG2a, kappa) to develop your recombinant rat IgG isotype controls（重组大鼠IgG同型对照）?
Answer: Our recombinant rat IgG isotype controls（重组大鼠IgG同型对照） including the rat IgG2b isotype control（大鼠IgG2b同型对照） (catalog # PA007144) are not from MOPC21 nor C1.18.4 though we also produce and sell the MOPC21 and C1.18.4 rat IgG isotype controls（大鼠IgG2b同型对照）. Please contact us and ask for MOPC21 and C1.18.4 derived recombinant rat IgG kappa isotype controls（重组大鼠IgG kappa同型对照） if you are interested.
其它体内实验级重组IgG同型对照抗体和突变体（in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants）:
体内实验级重组人IgG1同型对照抗体和突变体（In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants）
体内实验级重组人IgG2同型对照抗体（In vivo Grade Recombinant Human IgG2 Isotype Control Antibody）
体内实验级重组人IgG3同型对照抗体（In vivo Grade Recombinant Human IgG3 Isotype Control Antibody）
体内实验级重组人IgG4-S228P同型对照抗体和突变体（In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants）
体内实验级重组小鼠IgG1同型对照抗体和突变体（In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants）
体内实验级重组小鼠IgG2a同型对照抗体和突变体（In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants）
体内实验级重组小鼠IgG2b同型对照抗体和突变体（In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants）
体内实验级重组小鼠IgG2c同型对照抗体和突变体（In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants）
体内实验级重组小鼠IgG3同型对照抗体（In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody）
体内实验级重组大鼠IgG1同型对照抗体（In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody）
体内实验级重组大鼠IgG2a同型对照抗体（In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody）
体内实验级重组大鼠IgG2b同型对照抗体（In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）
体内实验级重组大鼠IgG2c同型对照抗体（In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody）
体内实验级重组仓鼠IgG1同型对照抗体（In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody）
体内实验级重组仓鼠IgG2同型对照抗体（In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody）
体内实验级重组IgG Fc蛋白（In vivo Grade Recombinant IgG Fc Proteins）:
体内实验级重组人IgG1 Fc蛋白(hIgG1)（In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)）
体内实验级重组人IgG2 Fc蛋白(hIgG2)（In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)）
体内实验级重组人IgG4 Fc蛋白(hIgG4)（In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)）
体内实验级重组小鼠IgG1 Fc蛋白(mIgG1)（In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)）
体内实验级重组小鼠IgG2a Fc蛋白(mIgG2a)（In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)）
体内实验级重组小鼠IgG2b Fc蛋白(mIgG2b)（In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)）
体内实验级重组大鼠IgG2a Fc蛋白(rtIgG2a)（In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)）
体内实验级重组大鼠IgG2b Fc蛋白(rtIgG2b)（In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)）
体内实验级重组羊驼IgG2b Fc蛋白(lIgG2b)（In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)）
体内实验级重组兔IgG Fc蛋白(rIgG)（In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)）
请记住我们的产品信息: 体内实验级重组大鼠IgG2b同型对照抗体: PA007144 Syd Labs （In Vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）。
关键词：体内实验级重组人IgG1-Fc蛋白（hIgG1）、体内实验级重组人IgG2-Fc蛋白（hIgG2）、体内实验级重组人IgG4 Fc蛋白（hIgG4）、体内实验级重组小鼠IgG1-Fc蛋白（mIgG1、体内实验级重组小鼠IgG2a-Fc蛋白（mIgG2a）、体内实验级重组小鼠IgG2b-Fc蛋白（mIgG2b）、体内实验级重组大鼠IgG2a-Fc蛋白（rtIgG2a）、体内实验级重组大鼠IgG2b-Fc蛋白（rtIgG2b）、体内实验级重组羊驼IgG2b-Fc蛋白（lIgG2b）、体内实验级重组兔IgG Fc蛋白（rIgG）、体内实验级重组兔IgG Fc蛋白（rIgG）、Spartalizumab生物类似药、Syd Labs华中代理、Syd Labs华北代理、Syd Labs华南代理、Syd Labs中国代理、Syd Labs北京代理
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