Syd Labs体内实验级重组小鼠IgG2a-N297A同型对照抗体（In Vivo Grade Recombinant Mouse IgG2a-N297A Isotype Control Antibody）。重组小鼠IgG2a kappa同型对照和突变体（Recombinant mouse IgG2a kappa isotype control and mutants），mIgG2a D265A, N297A和LALAPG，适合体外和体内研究。
和测试过的小鼠样本特异性结合低甚至无；更多重组小鼠IgG2a同型对照抗体（recombinant mouse IgG2a isotype controls）相关产品，包括各种靶标、突变体、标签和偶联物。
重点说明：此产品仅限于科研使用，不得用于人类或动物的诊断或治疗。
多找找 | Syd Labs体内实验级重组小鼠IgG2a-N297A同型对照抗体产品参数
货号：PA007137
名称：体内实验级重组小鼠IgG2a-N297A同型对照抗体
英文名：In Vivo Grade Recombinant Mouse IgG2a-N297A Isotype Control Antibody
供货商名称：Syd Labs, Inc.
公司名：Syd Labs
别称：mIgG2a同型对照抗体，mIgG2a D265A, mIgG2a N297A，小鼠IgG2a LALAPG同型对照突变体
概述：用哺乳动物细胞生产的体内实验级重组小鼠IgG2a-N297A同型对照抗体（In Vivo Grade Recombinant Mouse IgG2a-N297A Isotype Control Antibody）。
克隆号：12B9.
同种型：小鼠 IgG2a, kappa。
应用：ELISA、Western Blot (WB)、流式细胞术(Flow)、免疫沉淀(IP)、免疫组织化学(石蜡)(IHC (P))、免疫组织化学(Frozen) (IHC (F))和体内动物模型研究中对小鼠IgG2a N297A kappa抗体（mouse IgG2a N297A kappa antibodies）进行同型匹配阴性对照。
免疫源：N/A.
抗体形式：0.2 μM过滤溶液，1x PBS
内毒素：根据 LAL 方法，≤1 EU每1mg 蛋白质。提供特级体内实验级重组小鼠IgG2a-N297A同型对照抗体（In Vivo Grade Recombinant Mouse IgG2a-N297A Isotype Control Antibody，内毒素≤0.05 EU/mg)。
纯度：>95%（在还原条件下通过SDS-PAGE测定）
运输：Syd Labs体内实验级重组小鼠IgG2a-N297A同型对照抗体（In Vivo Grade Recombinant Mouse IgG2a-N297A Isotype Control Antibody）用冰袋运输。收到后，请立即将其存放在下面建议的温度下。
稳定性与存储：使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，如果保存在2 至 8°C。 自收到之日起 3 个月，如果保存在-20 至 -70°C。
注意事项：体内实验级重组人IgG1 kappa同型对照抗体和突变体（In Vivo Grade Recombinant Human IgG1 kappa Isotype Control Antibody mutant）;与经测试的人体样本的特异性结合程度低或无特异性结合;人源化可变区和人IgG1 kappa恒定区;可提供嵌合版本和优质级(内毒素≤0.05 EU/mg)。
PA007137：Syd Labs体内实验级重组小鼠IgG2a-N297A同型对照抗体（In Vivo Grade Recombinant Mouse IgG2a-N297A Isotype Control Antibody）
背景知识
Mouse IgG2a isotype control and mutants（小鼠IgG2a同型对照和突变体） (natural and engineered mIgG2a isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. Mouse IgG2a antibodies are similar to human IgG1 antibodies in many aspects, thus researchers like to use the isotype of mouse IgG2a in mouse studies. Most of the therapeutic antibody drugs carry the natural human IgG1 constant regions. More and more engineered mutants in the human IgG1 Fc fragment have been tested during therapeutic antibody drug development. The corresponding mutations have been introduced to mouse IgG2a to study enhancement and abrogation effects of Fc binding. We generated mIgG2a isotype control mutants（mIgG2a同型对照突变体）, the same mutations as those in the therapeutic antibodies and candidates because such mouse IgG2a isotype control mutants（小鼠IgG2a同型对照突变体） are needed when clients test their engineered antibody candidates. For example, P329G substitution combined with L234A L235A (mIgG2a LALAPG, Fc silenced) was shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface.
The ideal recombinant mouse IgG2a isotype control（重组小鼠IgG2a同型对照） has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG2a kappa constant regions. The mouse IgG2a kappa isotype control and mutants（小鼠IgG2a kappa同型对照和突变体） can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.
Please contact us to ask for a quote for the engineered mouse IgG2a isotype control mutants（小鼠IgG2a同型对照突变体）, mIgG2a isotype control and mutants（mIgG2a同型对照突变体） with Avi-, His-, and Flag-tags, and biotinylated mIgG2a isotype control and mutants（mIgG2a同型对照突变体）. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG2a isotype control and mutants（小鼠IgG2a同型对照和突变体） are available.
Mouse IgG antibodies（小鼠IgG抗体） are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.
Syd Labs体内实验级重组小鼠IgG2a-N297A同型对照抗相关问答:
Question: The monoclonal antibody produced by the hybridoma C1.18.4 (clone number) is used as a mouse IgG2a kappa isotype control（小鼠IgG2a kappa同型对照）. The antibody variable regions of MOPC21 (hybridoma clone number, isotype: mouse IgG1, kappa) are used to make a recombinant mouse IgG2a kappa isotype control（重组小鼠IgG2a kappa同型对照）. Both C1.18.4 and MOPC21 antibodies have unknown binding specificity. Is your isotype control the C1.18.4 or MOPC21 antibody?
Answer: Our recombinant mouse IgG1 isotype controls（重组小鼠IgG1同型对照） (catalog # PA007129 and the mutants) are not from C1.18.4 or MOPC21 though we also produce and sell the recombinant C1.18.4 and MOPC21 mouse IgG2a isotype controls（小鼠IgG2a同型对照）. Please contact us and ask for C1.18.4 and MOPC21 derived recombinant mouse IgG2a kappa isotype controls（重组小鼠IgG2a kappa同型对照） if you are interested.
Question: What is the advantage of the recombinant antibody production method compared to the other antibody production method(s)?
Answer: We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage of the in vitro method. First, we do not use mouse ascites for antibody production. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies. Fourth, we can easily switch the isotype with different antibody constant regions, and generate mutations such as D265A which is used in therapeutic antibody research. It is better to use the same isotype control mutation.
Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate any non-specific background signal from the specific antibody signal. Mouse IgG1 kappa isotype controls（小鼠IgG1 kappa同型对照） against KLH, TNP, DNP, GST, HEL, His-tag, Flag, c-myc, HA, and GFP meet the requirements in some cases but not in specific cases in the presence of the specific targets or cross-reactivity.
Syd Labs体内实验级重组小鼠IgG2a-N297A同型对照抗体: PA007137 Syd Labs （In Vivo Grade Recombinant Mouse IgG2a-N297A Isotype Control Antibody）更多的产品技术信息，来源于多找找科技。
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