Product Profile
Product Name
Annexin A1 antibody
Antibody Type
Primary Antibodies
Product description
More
Rabbit Polyclonal antibody to Annexin A1 (annexin A1)Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 ａnd are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins ａnd leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFIHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
HumanMouse
Predicted Reactivity
Rat, Dog, Cat, Pig, Rabbit, Chimpanzee, BovineRat (89%), Dog (92%), Cat (93%), Pig (87%), Rabbit (92%), Chimpanzee (99%), Bovine (89%)
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
More
ANX1 antibody
LPC1 antibody
ANXA1 antibody
calpactin-2 antibody
chromobindin-9 antibody
annexin-1 antibody
phospholipase A2 inhibitory protein antibody
calpactin II antibody
lipocortin I antibody
annexin I (lipocortin I) antibody
annexin A1 antib
Molecular Weight(MW)
39 kDa (note) (2)
Cellular Localization
Nucleus , Cylasm , Cell projection , cilium , Basolateral cell membrane
Application
Application
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Annexin A1 antibody at 1:500 dilution.
Application
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin A1 antibody at 1:200 dilution.
Application
- Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:10000
Application
- Wild-type (WT) ａnd Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, ａnd the membrane was blotted with Annexin A1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
- Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000
Positive Control
H1299 , NIH-3T3
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
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