Product Profile

Product Name

PPP2R4 antibody

Antibody Type

Primary Antibodies

Product description

More

Mouse Monoclonal Antibody [4D9] to PPP2R4Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Immunogen

The immunogen corresponding to human PPP2R4 expressed in E. Coli.

Key Feature

Clonality

Monoclonal

Isotype

IgG1

Clone Number

4D9

Host Species

Mouse

Tested Applications

ELISAFACSICC/IFIHCIHC-PWB

ELISA: 1/10000.:

Species Reactivity

Human

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Target Information

Gene Synonyms

PR53
PP2A
PTPA

Target

PPP2R4

Molecular Weight(MW)

41 kDa (note)

Application

打开网易新闻 查看精彩图片

Application

Application

  • ELISA analysis of control antigen and antigen using PPP2R4 antibody [4D9].

打开网易新闻 查看精彩图片

Application

Application

  • FACS analysis of MCF-7 cells using PPP2R4 antibody [4D9] (green) or a negative control (red).

打开网易新闻 查看精彩图片

Application

Application

  • ICC/IF analysis of HeLa cells using PPP2R4 antibody [4D9] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).

打开网易新闻 查看精彩图片

Application

Application

  • IHC-P analysis of cervical cancer tissues using PPP2R4 antibody [4D9].

打开网易新闻 查看精彩图片

Application

Application

  • IHC-P analysis of lung cancer tissues using PPP2R4 antibody [4D9].

打开网易新闻 查看精彩图片

Application

Application

  • WB analysis of human PPP2R4 (a.a. 1-154) recombinant protein using PPP2R4 antibody [4D9].

Application Notes

ELISA: 1/10000.:

Additional Information

Form

Liquid

Storage Instructions

4°C; -20°C for long term storage

Storage Buffer

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.