Product Profile
Product Name
BRCA1 [D2-C6]
Antibody Type
Primary Antibodies
Product description
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In 1990, a breast cancer susceptibility gene, designated BRCA1, was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45% of families with high incidence of breast cancer ａnd at least 80% of families with increased incidence of both early-onset breast cancer ａnd ovarian cancer. A second breast cancer susceptibility gene, BRCA2, located on chromosome 13q12-13, also confers a high incidence of breast cancer but, unlike BRCA1, does not confer a substantially elevated risk of ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast ａnd ovary, ａnd encodes a predicted protein of 1863 amino acids. This protein contains a zinc finger domain in its amino terminal region, but is otherwise unrelated to any previously described proteins. Like many other genes involved in familial cancer, BRCA1 appears to encode a tumor suppressor, a protein that acts as a negative regulator of tumor growth.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBIHC
WB:1:500-1:1,000
IHC:1:100-1:500
Species Reactivity
Human
Concentration
2 mg/mL.
Target Information
Alternative Names
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BRCA 1 antibody BRCA1 antibody BRCA1 DNA repair associated antibody BRCA1/BRCA2 containing complex subunit 1 antibody BRCA1/BRCA2-containing complex
subunit 1 antibody BRCA1_HUMAN antibody BRCAI antibody BRCC 1 antibody BRCC1 antibody Breast ａnd ovarian cancer susceptibility protein 1 antibody Breast Cancer 1 antibody Breast Cancer 1 Early Onset antibody Breast cancer type 1 susceptibility protein antibody BROVCA1 antibody FANCS antibody IRIS antibody PNCA4 antibody PPP1R53 antibody Protein phosphatase 1 regulatory subunit 53 antibody PSCP antibody RING finger protein 53 antibody RNF53 antibody
Molecular Weight(MW)
208 kDa
Cellular Localization
Cylasm. Nucleus. Plasma Membrane.
Database Links
SwissProt ID
P38398
Application
Application
- Fig1: Western blot analysis of BRCA1 on human BRCA1 recombinant protein using anti-BRCA1 antibody at 1/1,000 dilution.
Application
- Fig2: Western blot analysis of BRCA1 on HEK293 (1) ａnd BRCA1-hIgGFc transfected HEK293 (2) cell lysate using anti-BRCA1 antibody at 1/1,000 dilution.
Application
- Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-BRCA1 antibody. Counter stained with hematoxylin.
Application
- Fig4:Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-BRCA1 antibody. Counter stained with hematoxylin.
Positive Control
Human BRCA1 recombinant protein, BRCA1-hIgGFc transfected HEK293 cell lysate, human esophagus cancer tissue, human esophagus tissue.
Application Notes
WB:1:500-1:1,000
IHC:1:100-1:500
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
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